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. 2008 Jun 18;3(6):e2477. doi: 10.1371/journal.pone.0002477

Figure 5. α-Actinin is co-immunoprecipitated with FLAG-GNE in 293T-FG cells.

Figure 5

[A] Western blot analyses with anti-FLAG antibody (A1) and anti-α-actinin antibody (A2) on 5 µg lysate-samples of FLAG-GNE (FG)-transfected (T) and untransfected (UT) 293T cells, treated with SDS sample buffer in the presence (+) or absence (−) of β-ME. [B] Co-IP results. Western blot analyses with anti-FLAG antibody and anti-α-actinin antibody of IP eluates and 5 µg lysate-samples, of FLAG-GNE (FG)-transfected (T) and untransfected (UT) 293T cells.