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In vitro synthesis and specific immunoprecipitation of MCEs. (A) 35S-methionine-labeled proteins were synthesized in vitro by using luciferase (Luc) cDNA (lane 1) or cDNAs for wild type (lane 2), mutant (lane 3), and C-terminal fragment (Δ210, lane 4) of MCE. Products were analyzed by SDS/PAGE and autoradiography. (B) Products shown in A were immunoprecipitated with αMCE and analyzed by SDS/PAGE and autoradiography.
