Figure 7.
Complementation of S. cerevisiae lacking mRNA guanylyltransferase. Growth was measured on selective media using CEG1− cells transformed with empty parental pG-1 (vector), pG-1 containing wild-type (MCE) or mutant (K294A) MCE or with a CEG1-containing URA3 plasmid (CEG1) as detailed in Materials and Methods.