Figure 1.

Effects of sodium selenite on NF-κB DNA binding activity with Jurkat T cell nuclear extracts. (A) Nuclear extracts prepared from human Jurkat T cells preincubated with 0.1 nM human TNF-α at 37°C for 20 min were examined for NF-κB activation by electrophoretic mobility shift assay after treatments with various concentrations of selenite. Lanes: 1, 0 selenite; 2, 0 selenite with wild-type NF-κB consensus oligonucleotide; 3, 0 selenite with mutant NF-κB consensus oligonucleotide; 4, 1 μM selenite; 5, 3 μM selenite; 6, 5 μM selenite; 7, 7 μM selenite; 8, 10 μM selenite; 9, 10 μM selenite with 1 mM DTT; and 10, 10 μM selenite with 2 mM DTT. (B) Quantitative representation of A. The resulting autoradiograms of NF-κB-DNA complex were quantitated by PhosphoImager analysis.