Figure 3.

Selenite-mediated inhibition of NF-κB DNA binding and NO production. The human Jurkat T cells, JPX9, were preincubated overnight with bacterial LPS at 75 ng/ml and then treated with indicated concentrations of sodium selenite for 3 h. For analysis of NF-κB activation (•), nuclear extracts were prepared from untreated and LPS-treated cells, and NF-κB DNA binding was determined by electrophoretic mobility shift assays. The resulting autoradiograms of NF-κB-DNA complex were quantitated by PhosphorImager. For analysis of NO production (▪), the final products of NO in vivo (sum of both NO₂⁻ and NO₃⁻) in the culture media of untreated and LPS-treated cells were measured as described in Materials and Methods. ←LPS→ indicates the cultured cells that were retreated with bacterial LPS.