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. 2008 Jun 25;3(6):e2498. doi: 10.1371/journal.pone.0002498

Figure 1. Derivation of chondrogenically-committed cells from FF-hESCs.

Figure 1

(A) FF-BG02 hESCs maintained with MEF-conditioned medium supplemented with 4 ng/ml bFGF (bar = 100 µm) expressed Oct-4, SSEA-4, and Tra-180. (B) FF-hESCs were then co-cultured with chondrocytes in Transwell tissue culture for 3 weeks. (C) Upon stimulation, fibroblastic cells appeared (as indicated by actin phalloidin staining, red) and stained positive for both Type I collagen and Type II collagen (bar = 20 µm). (D) RT-PCR confirmed that chondrocyte co-culture resulted in upregulation of cartilage-specific markers including aggrecan, Type II collagen, and TGF-β-RII gene expressions and decreased expression of FLK1 and Myf5.