Table 1. Primer Sequences.
Gene | Primer Sequences (5′-3′) | Predicted size (bp) |
AGN | F-tgaggagggctggaacaagtacc | 350 |
R-ggaggtggtaattgcagggaaca | ||
Col I | F-tgacgagaccaagaactg | 600 |
R-ccatccaaaccactgaaacc | ||
Col II | F-tttcccaggtcaagatggtc | 377 |
R-cttcagcacctgtctcacca | ||
Nestin | F-gccctgaccactccagttta | 200 |
R-ggagtcctggatttccttcc | ||
flk-1 | F-ggtattggcagttggaggaa | 199 |
R-acatttgccgcttggataac | ||
myf5 | F-tcacctcctcagagcaacct | 193 |
R-tgaagccttcttcgtcctgt | ||
ALP | F-acgtggctaagaatgtcatc | 476 |
R-acatttgccgcttggataac | ||
Col X | F-ccctttttgctgctagtatcc | 468 |
R-ctgttgtccaggttttcctggcac | ||
COMP | F-caggacgactttgatgcaga | 314 |
R-aagctggagctgtcctggta | ||
link protein | F-gctctgtgcaatatcccatc | 232 |
R-cccacttttgcaatgtgagc | ||
Sox-9 | F-acgtcatctccaacatcgagacc | 4454 |
R-ctgtagtgtgggaggttgaaggg | ||
Cbfa1 | F-ccacccggccgaactggtcc | 258 |
R-cctcgtccgctccggcccaca | ||
BMP4-RII | F-tagtcactgacaacaacggtgcagtc | 816 |
R-tatactgctccatatcgacctcggc | ||
TGF-B-RII | F-tagtcactgacaacaacggtgcagtc | 539 |
R-acagtgctcgctgaactccatgagc | ||
TERT | F-agctatgcccggacctccat | 185 |
R-gcctgcagcaggaggatctt | ||
β-actin | F-tggcaccacaccttctacaatgagc | 396 |
R-gcacagcttctccttaatgtcacgc |