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. 2008 May 17;59(9):2309–2316. doi: 10.1093/jxb/ern095

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© 2008 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 5. — Phosphorylation of psToc34 in OEVs influences the Toc complex integrity. (A) Pea OEVs were solubilized with decylmaltoside after incubation with ³²P-ATP adjusted with ATP to 1 μM (final) and subjected to a 25–70% sucrose density gradient centrifugation. Fractions of the density region from 35–70% were collected and subjected to SDS-PAGE followed by silver staining (lower panel) and visualization of radioactivity (upper panel). The inset shows the distribution of phosphorylated psToc34 (squares) or psToc159_G/M (circles). (B) Pea OEVs were phosphorylated as described in the Materials and methods followed by immunoprecipitation using αToc75 or αOep37 antibodies. Flow through (10%, FT), wash (10%, W), and precipitate fractions (50%, E) were analysed by western blots (wb) using αToc159 (Toc159_GM) or αToc34 antibodies. Phosphorylation was visualized by autoradiography (P³²).