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. 2008 May 20;59(9):2545–2554. doi: 10.1093/jxb/ern123

Table 1.

Kinetic parameters for recombinant GR2

Varied substrate Chemical structure of varied substrate Fixed substrate Km (mM) Vmax (μmol min−1 mg−1 protein) kcat (s−1) kcat/Km (s−1 mM−1)
Glyoxylate graphic file with name jexbotern123fx1_ht.jpg NADPH 0.034±0.003 40.6±8.7 22.5±4.8 660±130
NADPH Glyoxylate 0.0014±0.0002 23.0±2.2 12.0±0.8 8550±960
SSA graphic file with name jexbotern123fx2_ht.jpg NADPH 8.96±0.71 30.7±4.0 17.0±2.2 1.89±0.27
NADPH SSA 0.0012±0.0002 19.3±1.5 10.7±0.8 9180±1620

Kinetic data for varied substrate concentrations were best fit by non-linear regression analysis. Parameters represent the mean ±SE of three independent enzyme preparations.