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. 2008 Jun;59(9):2529–2543. doi: 10.1093/jxb/ern119

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© 2008 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 7. — Analysis of FTIR spectra from pollen tubes of Torenia fournieri. (A, B) Average FTIR spectra obtained from the apical region (n=10) (A) and subapical region (n=6) (B) of pollen tubes cultured for 2 h under control conditions (CON) or treated with 4 mg l⁻¹ IAA (IAA), showing IAA treatment induced displacements of the peaks or changes in absorbance. (C, D) Typical score plots obtained from principal component analysis applied to the cell-wall spectra in the apical region (C) and subapical region (D) for the IAA-treated (IAA) and the control (CON) tubes. Best separation was obtained with the first principal component (PC1) score. (E, F) Difference spectra generated by digital subtraction of CON spectra from IAA spectra of the apical (E) or subapical region (F), showing that the content of proteins and pectin increased while the content of cellulose decreased after IAA treatment.