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Assessment of primary microglial cell culture purity by qRT–PCR
| Treatment | CD11b | GFAP |
|---|---|---|
| Unstimulated | 1.0 | ND |
| PGN, 6 h | 0.6 | ND |
| PGN, 12 h | 0.7 | ND |
| PGN, 24 h | 0.3 | ND |
The level of CD11b expression was calculated after normalizing target signals against GAPDH and is presented as the fold change in mRNA expression relative to unstimulated microglia. Results are representative of three independent experiments. ND, not detected.
