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. Author manuscript; available in PMC: 2008 Jun 10.
Published in final edited form as: J Neurochem. 2006 Dec;99(5):1389–1402. doi: 10.1111/j.1471-4159.2006.04183.x

Fig. 6.

Fig. 6

15d-PGJ2 and ciglitazone induce the expression of PPRE-responsive genes in astrocytes. Astrocytes were seeded in 6-well plates at 1 × 106 cells per well. After an overnight incubation, cells were treated with 20 μM of 15d-PGJ2 (15d) or 100 μM ciglitazone (cig) only. Total RNA was collected from cells at 6, 12 and 24 h following S. aureus stimulation, whereupon CD36 (a, c) and ABCA1 (b, d) expression was quantified by qRT-PCR as described in Materials and methods. Gene expression levels were calculated after normalizing target signals against the housekeeping gene GAPDH and are presented as relative mRNA expression units (mean ± SEM of three independent experiments). Significant differences between unstimulated versus 15d-PGJ2- or ciglitazone-treated astrocytes are denoted with asterisks (*p < 0.05, **p < 0.001).