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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Microbes Infect. 2007 Dec 23;10(3):302–312. doi: 10.1016/j.micinf.2007.12.007

Figure 1. Expression of IL-23p19 mRNA and protein in the corneas and lymphoid organs after HSV-1 ocular infection.

Figure 1

(A) WT mice were infected with 5 × 105 PFU of HSV-1 on their scarified corneas. At different time points, mRNA was extracted from the corneas and DLNs, and reverse transcribed into cDNA. Kinetic expression of IL-23p19 mRNA was measured by real-time PCR. Test samples were run in triplicate with a dilution series and the mean value of a particular dilution was used. Data are expressed as copy number per sample. (B) WT mice (n = 3) were ocularly infected with different doses of virus (5 × 105, 5 × 104, 5 × 105 PFU) and levels of IL-23 protein were estimated from lysate supernatants of corneas, DLNs, or spleen of mice by an antibody capture ELISA as described by materials and methods.