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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Microbes Infect. 2007 Dec 23;10(3):302–312. doi: 10.1016/j.micinf.2007.12.007

Figure 4. Increased population and number of IL-12 expressing cells in the lymphoid organs of p19-/- mice after HSV-1 infection.

Figure 4

At day 10 and 20 p.i., splenocytes or DLNs cells of the different mice groups (n = 4) were used in intracellular cytokine staining assay. Isolated splenocytes or DLNs cells from both groups having SK lesions were stimulated with anti-CD3 and anti-CD28 for 16 h. Single cells were extracellularly stained with anti-mouse CD11b (APC) and CD11c (PerCp) antibody and then intracellularly stained with anti-mouse IL-12 PE antibody (A and B). Values shown in each graph reflect the percentage or absolute number of spleen cells expressing IL-12.