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Growth curve and β-galactosidase activity of lacL8UV5 cells growing on a glucose–lactose medium. Cells were grown in M9 medium containing 0.04% glucose and 0.2% lactose. The following strains and addition were used: (A) PR166; (B) PR166 plus 0.5 mM isopropyl β-d-thiogalactoside; (C) KK21; and (D) PR166 harboring pIT539. At the indicated time, samples were removed to determine the OD (squares) and β-galactosidase activity (diamonds).
