Table 1.
Bacterial strains and plasmids used in this study
| Strain/plasmid | Relevant genotype and property | Source |
|---|---|---|
| Strain | ||
| W3110 | Wild-type | Laboratory stock |
| HT28 | W3110 Δcya::Kan | This study |
| IT1409 | W3110 Δcrp::Tet | This study |
| IT1168 | W3110 ptsG::Tn5 | This study |
| IT1199 | W3110 Δcrr::Kan | This study |
| PR158 | galK2 strR supo lacZYA21 F′plac+ lacZ+ | ref. 16 |
| PR166 | galK2 strR supo lacZYA21 F′placL8UV5lacZ+ | ref. 16 |
| KK8 | PR166 Δcya::Kan | This study |
| KK15 | PR158 Δcrp::Tet | This study |
| KK17 | PR166 Δcrp::Tet | This study |
| KK20 | PR166 ptsG::Tn5 | This study |
| KK21 | PR166 Δcrr::Kan | This study |
| Plasmid | ||
| pHA7 | Derivative of pBR322 containing the crp gene expressed from the bla promoter | ref. 18 |
| pIT302 | Derivative of pACYC184 containing the cya gene expressed from the bla promoter | This study |
| pTH111 | Derivative of pBR322 containing the ptsG gene expressed from bla promoter | This study |
| pST51 | Derivative of pBR322 containing the ptsHI genes expressed from the pts promoter | This study |
| pIT539 | Derivative of pBR322 containing the lacYA genes expressed from the bla promoter | This study |