Table 2.
lac promoter | crp | Strain | β-Galactosidase activity |
---|---|---|---|
L8UV5 | + | PR166 | 55 |
− | KK17 | 37 | |
Wild-type | + | PR158 | 26 |
− | KK15 | 6 |
Cells were grown at 37°C in Luria–Bertani medium to OD600 = 0.6. β-Galactosidase activity was determined and expressed in Miller units (17). Each value is the average of three experiments.