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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Mol Pharmacol. 2007 Dec 13;73(3):968–976. doi: 10.1124/mol.107.036780

Fig. 4.

Fig. 4

Effect of DHEA, nafenopin, and Wy-14643 on PPARα protein and mRNA expression in primary cultures of rat hepatocytes. A, cells were treated with different concentrations of DHEA, nafenopin and Wy-14643 for 48 h. Cellular protein (20 μg) were analyzed by SDS-PAGE and immunoblotting was performed using anti-PPARα antibody. Images of a representative immunoblot and bar graph of the quantification of scanned blots. B, cells were treated with different concentrations of DHEA, nafenopin, and Wy-14643 (WY) for 24 h. PPARα mRNA was measured by quantitative RT-PCR as described under Materials and Methods. Ribosomal 18S RNA levels were used as normalization standards. The values shown are expressed as the average and S.D. of six separate assays performed in triplicate.