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. 2008 Jun 12;118(7):2651–2660. doi: 10.1172/JCI34753

Figure 4. Silvestrol induces eIF4A association into RNAse-sensitive heavy sedimenting complexes in vivo.

Figure 4

(A) Effect of silvestrol on Jurkat cell polyribosomes. Jurkat cells were exposed to vehicle (MeOH) or 0.2 μM silvestrol for 60 minutes. Cell extracts were prepared and treated with micrococcal nuclease (MN) where indicated. Reactions were resolved on 10%–50% sucrose gradients by centrifugation in an SW40 rotor at 150,000 g for 2 hours. Fractions were collected from the gradients and monitored with an ISCO UA-6 UV detector. (B) Western blots demonstrating the position of migration of eIF4A, eIF4E, and eIF4G1 in sucrose fractions collected from untreated or MN-treated lysates prepared from cells exposed to MeOH or 0.2 μM silvestrol.