Figure 3.

Alignment of P4501α with closely related other CYPs. The closest relatives to P4501α were determined by using the blastp program provided by the National Center for Biotechnology Information, and the amino acid sequences were manually aligned to P4501α. CYP27, the predicted protein from the rat mitochondrial vitamin D₃-25-hydroxylase (GenBank accession no. M38566); CYP24, the predicted protein from the rat mitochondrial 25(OH)D₃-24-hydroxylase (GenBank accession no. X59506). Identical residues are shown in bold type, and dashes represent gaps for alignment purposes.