Figure 5.

Expression of CYP24 and P4501α mRNAs in rat kidney. Poly(A)⁺ RNAs (5 μg) were prepared from rats that had been treated with either 26,27-F₆-1α,25(OH)₂D₃ or vehicle. 26,27-F₆-1α,25(OH)₂D₃ (1 μg/rat) was intravenously administered to vitamin D-deficient low Ca rats. Rats were killed 48 h after administration of either 26,27-F₆-1α,25(OH)₂D₃ or vehicle. The blots were probed with [³²P]dCTP labeled P4501α (A) and CYP24 (B). Similar results were obtained in three independent sets of experiments.