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Expression of CYP24 and P4501α mRNAs in 3-week-old rats maintained for 2–3 weeks on a vitamin D-repleted diet containing 1.2% Ca and 0.6% P (Normal), 0.03% Ca and 0.6% P (Low Ca), or 1.2% Ca and 0.1% P (Low P). Poly(A)+ RNA (5 μg) was electrophoresed and probed for P4501α. Similar results were obtained in three independent sets of experiments.
