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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Exp Parasitol. 2007 Dec 3;118(4):629–636. doi: 10.1016/j.exppara.2007.11.013

Fig. 2. Western blot analyses of Leishmania single- and double-transfectants for delta-aminolevulinate dehydrase and porphobilinogen deaminase.

Fig. 2

Left and right panels, two representatives of the four Leishmania species examined. All lanes were loaded equally with proteins equivalent to ~107 cells grown to stationary phase and subjected to SDS-PAGE followed by immunoblotting. Lanes 1 and 8, alad/pbgd double transfectants; Lanes 2 and 7, single-transfectants with alad; Lanes 4 and 6, single-transfectants with pbgd; Lanes 3 and 5, wild-type cells. Note: the absence of both enzyme proteins in wild-type cells, and the presence of one or the other in the single transfectants and both only in the double transfectants. pX was used for alad and p6.5 for pbgd expression in all cases.