Figure 2. Quantitative PCR of upregulated genes following Trsp removal in liver.
The relative expression of genes upregulated in ΔTrsp mice (Table 3) was examined by Q-PCR, normalized to the expression of Gusb as described in Experimental Procedures. The normalized value for each mRNA in liver of ΔTrsp, A34, G37L and G37H mice was then compared to Trsp mice and plotted along with error bars. Upregulated genes analyzed are grouped according to their function (see Table 3): (A) metabolism; (B) defense stress and detoxification; (C) intracellular communication/signal transduction; and (D) cell cycle/growth and differentiation. Results represent 3-4 independent experiments, each carried out in triplicate.