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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Biochem J. 2008 Jul 1;413(1):151–161. doi: 10.1042/BJ20080277

Figure 3. Western blot analysis of phase II enzymes.

Figure 3

Protein extracts were prepared from liver of Trsp, ΔTrsp, A34, G37L and G37H mice and electrophoresed on 10% polyacrylamide gels. (A) Coomassie Blue stained gel. The elevated band corresponding to GST in ΔTrsp mice is indicated by an arrow on the right side of the gel and molecular weight (MW) markers were run in lane 1 and their sizes indicated on the left. (B) The proteins were transferred to PVDF membranes and the membranes probed with antibodies specific for the indicated phase II enzymes and β-actin as described in Experimental Procedures. β-Actin served as a loading control.

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