Table 3.
Transcripts in elevated levels within liver of knockout mice relative to wild type mice a
| ΔTrsp vs Trsp | A34 vs Trsp | G37L vs Trsp | G37H vs Trsp | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| UniGene | Description | Folds | P value | Folds | P value | Folds | P value | Folds | P value | Function/Gene Ontology |
| Defense stress and detoxification b | ||||||||||
| Mm.28191 | carboxylesterase 2 (Ces2) | 3.8 | 0.02 | -0.7 | 0.07 | -0.5 | 0.17 | 0.5 | 0.53 | Ester hydrolase activity |
| Mm.389848 | Cytochrome P450, family 2, subfamily a, polypeptide 5 (Cyp2a5) | 8.2 | 0.01 | 3.0 | 0.30 | -5.6 | 0.03 | 0.2 | 0.51 | Degradation of environmental toxins and mutagens |
| Mm.32550 | DnaJ (Hsp40) homolog, subfamily C, member 12 (Dnajc12) | 2.2 | 0.00 | 0.4 | 0.51 | 5.5 | 0.11 | -1.5 | 0.00 | Heat shock protein binding, protein folding |
| Mm.218639 | sulfiredoxin 1 homolog (S. cerevisiae) (Srxn1) | 3.3 | 0.03 | -0.2 | 0.19 | 0.9 | 0.96 | -1.6 | 0.00 | Antioxidant activity, response to oxidative stress |
| Metabolism b | ||||||||||
| Mm.30085 | aldo-keto reductase family 1, member A4 (aldehyde reductase) (Akr1a4) | 2.4 | 0.00 | 1.3 | 0.03 | -0.1 | 0.18 | 1.1 | 0.01 | Glycerolipid metabolism |
| Mm.26787 | aldehyde oxidase 1 (Aox1) | 6.5 | 0.01 | 0.7 | 0.73 | -1.8 | 0.00 | -1.1 | 0.00 | Xenobiotic metabolism |
| Mm.24021 | Biliverdin reductase B (Blvrb) | 3.4 | 0.01 | -1.5 | 0.00 | -2.1 | 0.00 | -1.7 | 0.00 | Porphyrin and chlorophyll metabolism |
| Mm.22720 | carboxylesterase 1 (Ces1) | 8.0 | 0.01 | 2.8 | 0.09 | -2.4 | 0.00 | 3.0 | 0.00 | Alkaloid biosynthesis |
| Mm.12906 | dopa decarboxylase (Ddc) | 5.1 | 0.00 | -0.5 | 0.15 | -2.0 | 0.15 | -1.1 | 0.00 | Amino acid and derivative metabolic process |
| Mm.22758 | emopamil binding protein-like (Ebpl) | 2.2 | 0.00 | 0.6 | 0.48 | 1.2 | 0.09 | 1.3 | 0.00 | Sterol metabolism |
| Mm.10211 | ectonucleoside triphosphate diphosphohydrolase 5, transcript variant 1 (Entpd5) | 5.4 | 0.02 | -0.7 | 0.03 | -2.3 | 0.00 | -1.6 | 0.00 | Purine metabolism; pyrimidine metabolism |
| Mm.9075 | epoxide hydrolase 1, microsomal (Ephx1) | 3.3 | 0.00 | 1.5 | 0.02 | -1.8 | 0.00 | -0.3 | 0.14 | Xenobiotic metabolism |
| Mm.252391 | glycerol-3-phosphate dehydrogenase 1 (soluble) (Gpd1) | 3.1 | 0.02 | -0.1 | 0.24 | 1.4 | 0.02 | 1.2 | 0.08 | Carbohydrate metabolism |
| Mm.283573 | Glutathione reductase 1 (Gsr) | 3.2 | 0.00 | 0.2 | 0.42 | 1.2 | 0.02 | -1.1 | 0.00 | Glutathione metabolism |
| Mm.197422 | Glutathione S-transferase, alpha 1 (Ya) (Gsta1) | 23.4 | 0.00 | -0.2 | 0.33 | -3.6 | 0.00 | -0.3 | 0.14 | Glutathione metabolism; xenobiotic metabolism |
| Mm.422778 | Glutathione S-transferase, alpha 2 (Yc2) (Gsta2) | 2.9 | 0.001 | 0.45 | 0.55 | -2.3 | 0.06 | 1.2 | 0.00 | Glutathione metabolism; xenobiotic metabolism |
| Mm.2662 | Glutathione S-transferase, alpha 4 (Gsta4) | 8.6 | 0.00 | 0.6 | 0.51 | -1.5 | 0.00 | 1.8 | 0.05 | Glutathione metabolism; xenobiotic metabolism |
| Mm.37199 | Glutathione S-transferase, mu 1 (Gstm1) | 3.2 | 0.01 | 1.4 | 0.00 | 0.0 | 0.20 | 1.5 | 0.10 | Glutathione metabolism; xenobiotic metabolism |
| Mm.37199 | Glutathione S-transferase, mu 2 (Gstm2) | 4.8 | 0.01 | -0.6 | 0.03 | -1.2 | 0.00 | -1.8 | 0.00 | Glutathione metabolism; xenobiotic metabolism |
| Mm.37199 | Glutathione S-transferase, mu 3 (Gstm3) | 12.0 | 0.01 | 0.4 | 0.54 | 0.0 | 0.16 | 1.2 | 0.21 | Glutathione metabolism; xenobiotic metabolism |
| Mm.5731 | Glutathione S-transferase, theta 3 (Gstt3) | 5.1 | 0.04 | -1.8 | 0.07 | -2.4 | 0.15 | 1.3 | 0.01 | Glutathione metabolism |
| Mm.276389 | heme oxygenase (decycling) 1 (Hmox1) | 17.3 | 0.00 | 1.6 | 0.08 | 2.4 | 0.00 | 2.2 | 0.02 | Porphyrin and chlorophyll metabolism; xenobiotic metabolism |
| Mm.299381 | hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1) | 2.8 | 0.00 | 0.6 | 0.54 | 1.3 | 0.00 | 1.1 | 0.12 | Purine metabolism |
| Mm.218286 | microsomal glutathione S-transferase 3 (Mgst3) | 5.8 | 0.00 | -0.5 | 0.05 | -2.7 | 0.00 | 1.1 | 0.04 | Glutathione metabolism; xenobiotic metabolism |
| Mm.344831 | UDP-glucose dehydrogenase (Ugdh) | 6.4 | 0.002 | 0.5 | 0.56 | -0.1 | 0.16 | -1.2 | 0.00 | Nucleotide sugar metabolism; starch and sucrose metabolism |
| Mm.312095 | UDP glucuronosyltransferase 2 family, polypeptide B35 (Ugt2b35) | 5.0 | 0.01 | 0.6 | 0.61 | -2.4 | 0.00 | -1.4 | 0.00 | Sugar metabolism |
| Intracellular communication/signal transduction b | ||||||||||
| Mm.18628 | CD36 antigen (Cd36) | 5.6 | 0.00 | -2.6 | 0.00 | -3.2 | 0.00 | -3.8 | 0.00 | Adipocytokine signaling pathway; PPAR signaling pathway |
| Mm.6529 | Dystrophia myotonica-protein kinase (Dmpk) | 5.0 | 0.00 | 1.0 | 0.97 | 1.3 | 0.06 | 1.5 | 0.00 | Protein amino acid phosphorylation; regulation of small GTPase mediated signal transduction |
| Mm.12967 | inhibitor of kappaB kinase gamma, transcript variant 2 (Ikbkg) | 5.1 | 0.02 | 1.2 | 0.01 | -1.6 | 0.00 | -1.1 | 0.00 | Activation of NF-kappaB-inducing kinase |
| Mm.294007 | PREDICTED: proprotein convertase subtilisin/kexin type 6, transcript variant 4 (Pcsk6) | 3.7 | 0.00 | 0.9 | 0.94 | -0.7 | 0.03 | 1.8 | 0.04 | Determination of left/right symmetry; transmembrane receptor protein tyrosine kinase signaling pathway |
| Mm.308180 | protein tyrosine phosphatase-like A domain containing 1 (Ptplad1) | 3.0 | 0.00 | 0.7 | 0.64 | 1.6 | 0.09 | 0.3 | 0.41 | I-kappaB kinase/NF-kappaB cascade; JNK cascade; Rho protein signal transduction |
| Cell cycle/Growth and differentiation b | ||||||||||
| Mm.20801 | HIV-1 tat interactive protein 2, homolog (human) (Htatip2) | 4.2 | 0.01 | -0.1 | 0.22 | -0.7 | 0.03 | 0.4 | 0.40 | Regulation of angiogenesis and apoptosis; cell differentiation |
| Mm.43831 | lectin, galactose binding, soluble 1 (Lgals1) | 6.0 | 0.01 | -1.4 | 0.03 | -1.1 | 0.00 | -1.5 | 0.00 | Myoblast differentiation; sugar binding |
| Mm.62876 | ZW10 interactor (Zwint) | 3.0 | 0.01 | -0.5 | 0.04 | -1.2 | 0.00 | 0.3 | 0.43 | cell cycle; cell division |
| Mm.290692 | transketolase (Tkt) | 2.6 | 0.00 | -0.5 | 0.03 | 0.5 | 0.40 | -1.2 | 0.00 | Metal ion binding; regulation of growth |
| Cellular transport and transport mechanism b | ||||||||||
| Mm.103777 | Rhesus blood group-associated B glycoprotein (Rhbg) | 4.8 | 0.02 | 0.1 | 0.24 | -0.8 | 0.03 | -1.1 | 0.00 | Ammonium transporter activity |
| Mm.222536 | solute carrier family 25 (mitochondrial carrier, peroxisomal membrane protein), member 17 (Slc25a17) | 2.4 | 0.00 | -0.9 | 0.02 | -1.9 | 0.00 | -5.8 | 0.00 | mitochondrial transport; transporter activity |
| Mm.281800 | Solute carrier family 35 (UDP-glucuronic acid/UDP-N-acetylgalactosamine dual transporter), member D1 (Slc35d1) | 2.8 | 0.00 | 0.6 | 0.53 | 0.5 | 0.37 | -1.2 | 0.00 | Nucleotide-sugar transporter |
| Mm.218875 | target of myb1-like 2 (chicken), transcript variant 1 (Tom1l2) | 2.1 | 0.00 | -2.1 | 0.06 | 5.4 | 0.00 | 2.5 | 0.01 | Golgi vesicle-mediated transport; intracellular protein transport |
| Transcription/Translation/Protein modification b | ||||||||||
| Mm.298030 | synovial sarcoma, X member B, breakpoint 1 (Ssxb1) | 5.1 | 0.02 | -0.2 | 0.20 | 1.7 | 0.05 | -1.4 | 0.00 | Regulation of transcription |
| Mm.34483 | leucine rich repeat containing 35 (Lrrc35) | 3.2 | 0.00 | 1.4 | 0.03 | -0.1 | 0.25 | -0.4 | 0.12 | Metal ion binding; protein modification |
| Miscellaneous (mixed functions) b | ||||||||||
| Mm.234247 | Dimethylarginine dimethylaminohydrolase 1 (Ddah1) | 4.5 | 0.01 | -0.6 | 0.08 | 0.2 | 0.33 | 1.4 | 0.28 | nitric oxide biosynthesis; protein amino acid nitrosylation |
| Mm.26834 | EF hand domain family A1 (Efha1) | 2.4 | 0.00 | -1.3 | 0.00 | -0.4 | 0.15 | -1.3 | 0.00 | Calcium ion binding |
| Mm.182912 | growth hormone inducible transmembrane protein (Ghitm) | 2.2 | 0.00 | -0.8 | 0.07 | 1.1 | 0.09 | 1.1 | 0.10 | Interacts with FtsH |
| Mm.41665 | Glutamate receptor, ionotropic, N-methyl D-asparate-associated protein 1 (glutamate binding) (Grina) | 2.3 | 0.00 | 0.1 | 0.29 | 0.7 | 0.69 | 0.5 | 0.54 | Receptor activity |
| Mm.228797 | major vault protein (Mvp) | 3.2 | 0.01 | 0.6 | 0.57 | 1.5 | 0.62 | 1.1 | 0.00 | Calcium ion binding; ribonucleoprotein complex |
| Mm.252080 | PREDICTED: phosphogluconate dehydrogenase, transcript variant 1 (Pgd) | 3.0 | 0.00 | -2.0 | 0.00 | 0.5 | 0.45 | -1.2 | 0.00 | pentose-phosphate shunt, oxidative branch |
| Mm.293463 | pirin (Pir) | 2.2 | 0.05 | 1.2 | 0.01 | -0.1 | 0.18 | 0.2 | 0.46 | metal ion binding |
| Mm.173058 | secreted phosphoprotein 2 (Spp2) | 2.3 | 0.00 | 1.3 | 0.01 | 0.8 | 0.74 | 1.7 | 0.00 | Bone remodeling |
| Unknown b | ||||||||||
| Mm.22109 | RIKEN cDNA 2610204L23 gene (2610204L23Rik) | 2.3 | 0.00 | 0.2 | 0.34 | 1.6 | 0.01 | -0.8 | 0.14 | Unknown |
| Mm.100125 | SH3 domain binding glutamic acid-rich protein like 2 (Sh3bgrl2) | 3.5 | 0.03 | 0.8 | 0.85 | 0.6 | 0.55 | 0.5 | 0.62 | Unknown |
| Mm.102407 | testis expressed gene 2 (Tex2) | 2.4 | 0.00 | 0.7 | 0.61 | 0.0 | 0.20 | 0.3 | 0.39 | Unknown |
| Mm.289795 | ubiquitin-associated protein 1 (Ubap1) | 2.6 | 0.00 | 0.5 | 0.44 | 0.6 | 0.43 | -1.2 | 0.00 | Unknown |
| Mm.257762 | PREDICTED: WD repeat domain 43, transcript variant 9 (Wdr43) | 4.1 | 0.00 | 0.6 | 0.43 | -0.8 | 0.03 | 1.3 | 0.04 | Unknown |
a
Genes elevated ≥ 2.0 fold in ΔTrsp mice compared to Trsp mice with a P-value ≤ 0.05 were assessed and shown in the table. The corresponding transcript levels were also analyzed in A34, G37L and G37H replacement mice relative to Trsp mice. Gene Unigene Accession Number, gene description, fold change with P value, and gene function(s) are shown. Four control and four experimental animals were used for comparing ΔTrsp, A34 and G37L with Trsp, while three control and three experimental animals were used for comparing G37H with Trsp.
b
Genes are grouped into classes according to function.