Table 4.
Transcripts in reduced levels within liver of knockout mice relative to wild type mice a
| ΔTrsp vs Trsp | A34 vs Trsp | G37L vs Trsp | G37H vs Trsp | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| UniGene | Description | Folds | P value | Folds | P value | Folds | P value | Folds | P value | Function/Gene Ontology |
| Cellular transport and transport mechanism b | ||||||||||
| Mm.381860 | coatomer protein complex, subunit gamma 2 (Copg2) | -2.3 | 0.00 | -0.8 | 0.05 | -2.5 | 0.00 | -1.7 | 0.00 | Protein transport |
| Mm.261168 | Potassium inwardly-rectifying channel, subfamily J, member 9 (Kcnj9) | -2.3 | 0.00 | -0.5 | 0.08 | 1.1 | 0.01 | -1.7 | 0.00 | Ion transport; voltage-gated ion channel activity |
| Mm.283370 | Neurocalcin delta (Ncald) | -2.3 | 0.00 | -1.5 | 0.00 | 1.5 | 0.00 | 1.2 | 0.00 | Vesicle-mediated transport |
| Mm.296837 | sideroflexin 2 (Sfxn2) | -3.8 | 0.01 | 0.1 | 0.49 | -1.4 | 0.05 | 0.0 | 0.43 | Ion transport |
| Mm.250738 | solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 3 (Slc13a3) | -2.4 | 0.00 | -0.8 | 0.04 | -1.2 | 0.00 | 1.0 | 0.00 | Dicarboxylic acid transport; ion transport; symporter activity; |
| Mm.255586 | solute carrier organic anion transporter family, member 1a4 (Slco1a4) | -2.5 | 0.00 | 1.0 | 0.98 | -0.3 | 0.20 | -1.2 | 0.00 | Ion transport; organic anion transporter activity |
| Intracellular communication/Signal transduction b | ||||||||||
| Mm.376094 | Anti-Mullerian hormone (Amh) | -2.3 | 0.00 | -0.0 | 0.41 | -0.1 | 0.24 | -1.0 | 0.32 | Transforming growth factor beta receptor binding |
| Mm.271854 | Low density lipoprotein receptor-related protein 1 (Lrp1) | -2.3 | 0.00 | -0.1 | 0.23 | 0.0 | 0.18 | -1.3 | 0.00 | Negative regulation of Wnt receptor signaling pathway |
| Mm.6595 | mitogen activated protein kinase kinase kinase 5 (Map3k5) | -2.3 | 0.00 | -0.2 | 0.35 | 1.2 | 0.00 | 0.1 | 0.43 | Activation of MAPK activity |
| Mm.3810 | phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide (Pik3c2a) | -2.2 | 0.00 | 0.4 | 0.58 | -0.7 | 0.23 | 1.6 | 0.00 | Intracellular signaling cascade |
| Metabolism b | ||||||||||
| Mm.46269 | insulin I (Ins1) | -2.3 | 0.00 | 1.7 | 0.02 | -1.1 | 0.00 | 2.1 | 0.01 | Carbohydrate metabolism |
| Mm.34459 | junctophilin 2 (Jph2) | -2.7 | 0.00 | 0.3 | 0.34 | -1.8 | 0.00 | -1.2 | 0.00 | Carbohydrate metabolism |
| Mm.19669 | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3) | -2.3 | 0.05 | -2.3 | 0.19 | 0.7 | 0.60 | 2.1 | 0.01 | Fructose and mannose metabolism |
| Mm.9277 | phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) (Pla2g7) | -2.8 | 0.00 | 2.2 | 0.14 | 0.7 | 0.60 | 1.1 | 0.03 | Lipid catabolism |
| Cellular structural organization b | ||||||||||
| Mm.49994 | hook homolog 1 (Drosophila) (Hook1) | -3.1 | 0.00 | 0.4 | 0.59 | 0.0 | 0.19 | -1.1 | 0.00 | Cytoskeleton organization and biogenesis; spermatid development |
| Mm.129840 | phosphodiesterase 4D interacting protein transcript variant 1 (Pde4dip), | -2.4 | 0.00 | 0.5 | 0.56 | -1.2 | 0.00 | 1.0 | 0.21 | Association with spindle pole body microtubules |
| Mm.9684 | vasodilator-stimulated phosphoprotein (Vasp) | -2.0 | 0.00 | 1.2 | 0.01 | -1.2 | 0.00 | -1.1 | 0.00 | Actin cytoskeleton organization and biogenesis |
| Transcription/Translation b | ||||||||||
| Mm.174044 | RIKEN cDNA 2700050L05 gene, transcript variant 2 (2700050L05Rik) | -2.0 | 0.00 | -1.2 | 0.00 | -1.4 | 0.00 | -1.8 | 0.00 | Transcription regulation |
| Mm.2645 | eukaryotic translation elongation factor 1 alpha 2 (Eef1a2) | -2.4 | 0.00 | -2.4 | 0.02 | -1.8 | 0.00 | 1.3 | 0.03 | Protein biosynthesis; translational elongation |
| Mm.303355 | One cut domain, family member 1 (Onecut1) | -4.0 | 0.04 | -1.8 | 0.09 | -2.1 | 0.00 | -2.5 | 0.00 | Regulation of transcription |
| Mm.7916 | Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) (Paf1) | -2.7 | 0.00 | 0.7 | 0.77 | -0.9 | 0.16 | 0.0 | 0.31 | Regulation of transcription |
| Mm.244820 | TATA box binding protein (Tbp) | -2.6 | 0.00 | 0.4 | 0.45 | 1.2 | 0.00 | -1.4 | 0.00 | Regulation of transcription |
| Miscellaneous (Mixed functions) b | ||||||||||
| Mm.28796 | RIKEN cDNA 5730446C15 gene, (5730446C15Rik) | -2.1 | 0.00 | 0.7 | 0.74 | -0.5 | 0.24 | 1.7 | 0.00 | Peptidase activity |
| Mm.71924 | ankyrin repeat domain 38 (Ankrd38) | -2.3 | 0.00 | -0.7 | 0.01 | -1.3 | 0.00 | -1.1 | 0.00 | Protein-protein interaction |
| Mm.119936 | attractin (Atrn) | -2.0 | 0.00 | 2.2 | 0.01 | -1.1 | 0.00 | -1.7 | 0.00 | Inflammatory response; protein binding; sugar binding |
| Mm.212861 | cullin 4A (Cul4a) | -2.2 | 0.00 | 1.7 | 0.62 | -1.4 | 0.00 | -1.1 | 0.00 | Cell cycle; induction of apoptosis by intracellular signals; ubiquitin cycle |
| Mm.171323 | nuclear receptor coactivator 6 interacting protein (Ncoa6ip) | -2.4 | 0.00 | -0.8 | 0.03 | -0.4 | 0.17 | -0.6 | 0.14 | S-adenosylmethionine-dependent methyltransferase activity; protein binding |
| Mm.261818 | Ring finger protein 167 (Rnf167) | -2.8 | 0.00 | 1.2 | 0.91 | -1.5 | 0.00 | -1.5 | 0.00 | Metal ion binding; protein binding; peptidase activity |
| Mm.284592 | SUMO/sentrin specific peptidase 3 (Senp3) | -2.2 | 0.00 | 0.2 | 0.35 | -0.6 | 0.04 | 0.3 | 0.43 | Peptidase activity; protein metabolism; ubiquitin-specific protease activity |
| Unknown b | ||||||||||
| Mm.416885 | RIKEN cDNA 2010109N18 gene | -2.9 | 0.00 | 0.3 | 0.36 | 3.5 | 0.27 | -1.4 | 0.00 | Unknown |
| Mm.138091 | PREDICTED: RIKEN cDNA 3110001I20 gene, transcript variant 1 (3110001I20Rik) | -2.3 | 0.00 | -0.9 | 0.05 | -1.7 | 0.10 | -0.9 | 0.13 | Unknown |
| Mm.395958 | RIKEN cDNA 9130221J18 gene | -4.9 | 0.00 | -0.3 | 0.14 | 0.3 | 0.52 | 1.4 | 0.01 | Unknown |
| Mm.395042 | RIKEN cDNA 9430078K24 gene | -2.1 | 0.00 | -0.3 | 0.13 | -1.2 | 0.00 | 1.8 | 0.00 | Unknown |
| Mm.425114 | PREDICTED: similar to gonadotropin inducible ovarian transcription factor 1, transcript variant 3 (LOC664985) | -2.9 | 0.00 | 0.2 | 0.35 | 0.1 | 0.24 | -1.2 | 0.00 | Unknown |
| Mm.219946 | SET and MYND domain containing 5 (Smyd5) | -2.7 | 0.00 | 0.0 | 0.21 | -1.1 | 0.00 | -2.0 | 0.00 | Unknown |
a
Genes depleted ≥ 2.0 fold in ΔTrsp mice as compared to Trsp mice with a P-value ≤ 0.05 were assessed and shown in the table. The corresponding transcript levels were also analyzed in A34, G37L and G37H replacement mice relative to Trsp mice. Gene Unigene Accession Number, gene description, fold change with P value, and gene function(s) are shown. Four control and four experimental animals were used for comparing ΔTrsp, A34 and G37L with Trsp, while three control and three experimental animals were used for comparing G37H with Trsp.
b
Genes are grouped into classes according to function.