TABLE 1.

Biophysical properties of MutSα and PCNA derived from SAXS and gel filtration

R_g and D_max were derived from SAXS data, and Stokes radii were determined by gel filtration (see “Experimental Procedures”). Calculated R_g values were determined from crystal structures (44). D_max values shown in parentheses were calculated from the corresponding crystal structures (44). The C termini of MutSαΔ341 (~80 residues) are not well ordered in the crystal structure and are therefore not included in the calculations.

Sample	Molecular mass	SAXS				Gel filtration, Stokes radius
		Rg calculated	Rg Guinier	RgP(r)	Dmax
	kDa		Å			Å
MutSα	258		59 ± 1	57 ± 0.2	202	67
MutSαΔ12	256		57 ± 0.4	58 ± 0.1	199	67
MutSαΔ341	220		46 ± 0.3	46 ± 0.1	145	60
MutSαΔ341·G-T	230	42	44 ± 0.1	43 ± 0.1	140 (130)
PCNA	86	34	33 ± 0.1	34 ± 0.1	92 (97)	40
MutSα + PCNA (1:1)	343		69 ± 0.5	67 ± 0.3	234	80