Skip to main content
. Author manuscript; available in PMC: 2008 Jun 10.
Published in final edited form as: Mol Cell. 2006 May 19;22(4):501–510. doi: 10.1016/j.molcel.2006.04.023

Figure 3.

Figure 3

MutSα Binds to O6-meG/T Mispairs Gel mobility shift assays were performed with 41 bp DNA duplexes containing G/C, G/T, O6-meG/C, or O6-meG/T base pairs. 32P 5′ labeled DNA was incubated with the indicated concentration of purified recombinant MutSα and subjected to native gel electrophoresis. Concentrations of MutSα that gave half-maximal binding were 10 ± 0.9 nM for O6-meG/T, 10 ± 1.1 nM for G/T, and >100 nM for O6-meG/C and G/C. Error bars represent the SDs of three independent experiments.