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Published in final edited form as: Cell Signal. 2007 Dec 8;20(4):675–683. doi: 10.1016/j.cellsig.2007.12.003

(A) SB202190 induces phosphorylation of JNK in human microvascular endothelial cells (HMVEC) HMVEC cells were treated with DMSO control or with 10 µM of SB202190 for 0.5, 1, 1.5, 2, 3, 4, or 6 hours, followed by detection of pJNK or JNK as described in the Methods section. (B) SB202190 induces phosphorylation of JNK in MCF-7 cells. MCF-7 cells were treated with SB202190 (5µM) for 0.5, 1, 2, 4, or 6 hours, and pJNK or JNK was detected as mentioned in the Methods section (lanes 1–6). In lane 7, MCF-7 cells were pretreated with 5 µM of SP600125 for 2 hours, followed by treatment with 5 µM of SB202190, and incubated for another 2 hours, followed by PJNK and JNK detection. In lane 8, cells were treated with anisomycin (1 µg/ml) for 2 hours as a positive control.

(A) SB202190 induces phosphorylation of JNK in human microvascular endothelial cells (HMVEC) HMVEC cells were treated with DMSO control or with 10 µM of SB202190 for 0.5, 1, 1.5, 2, 3, 4, or 6 hours, followed by detection of pJNK or JNK as described in the Methods section. (B) SB202190 induces phosphorylation of JNK in MCF-7 cells. MCF-7 cells were treated with SB202190 (5µM) for 0.5, 1, 2, 4, or 6 hours, and pJNK or JNK was detected as mentioned in the Methods section (lanes 1–6). In lane 7, MCF-7 cells were pretreated with 5 µM of SP600125 for 2 hours, followed by treatment with 5 µM of SB202190, and incubated for another 2 hours, followed by PJNK and JNK detection. In lane 8, cells were treated with anisomycin (1 µg/ml) for 2 hours as a positive control.