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. Author manuscript; available in PMC: 2009 Apr 1.

Published in final edited form as: Cell Signal. 2007 Dec 8;20(4):675–683. doi: 10.1016/j.cellsig.2007.12.003

(A) Effect of SB202190 on MLK3 phosphorylation. HEK 293T cells were transfected with phCMV2-HA-MLK3 as described in the methods. After 48 hours cells were treated with SB202190 for 4 hours. Cellular lysates were probed with anti-phospho-MLK3 antibody as described in the methods. Lane 1, DMSO treated control; lane 2, 5 µM SB202190; Lane3, 10 µM SB202190; Bottom Panel, Flag-MLK3; Lower Panel, β-actin. (B) Effect of SB203580 on MLK3 phosphorylation. HEK 293T cells were transfected with pCMV2-HA-MLK3 as described in the methods. After 48 hours cells were treated with SB203580 for 4 hours. Cellular lysates were probed with anti-phospho-MLK3 antibody as described in the methods. Lane 1, DMSO treated control; lane 2, 5 µM SB203580; Lane3, 10 µM SB203580; Bottom Panel, Flag-MLK3; Lower Panel, β-actin.

(A) Effect of SB202190 on MLK3 phosphorylation. HEK 293T cells were transfected with phCMV2-HA-MLK3 as described in the methods. After 48 hours cells were treated with SB202190 for 4 hours. Cellular lysates were probed with anti-phospho-MLK3 antibody as described in the methods. Lane 1, DMSO treated control; lane 2, 5 µM SB202190; Lane3, 10 µM SB202190; Bottom Panel, Flag-MLK3; Lower Panel, β-actin. (B) Effect of SB203580 on MLK3 phosphorylation. HEK 293T cells were transfected with pCMV2-HA-MLK3 as described in the methods. After 48 hours cells were treated with SB203580 for 4 hours. Cellular lysates were probed with anti-phospho-MLK3 antibody as described in the methods. Lane 1, DMSO treated control; lane 2, 5 µM SB203580; Lane3, 10 µM SB203580; Bottom Panel, Flag-MLK3; Lower Panel, β-actin.