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. Author manuscript; available in PMC: 2009 Mar 13.
Published in final edited form as: Cell Host Microbe. 2008 Mar 13;3(3):137–145. doi: 10.1016/j.chom.2008.02.003

Figure 6. Killing of Mtb by D157070 Is DlaT-Dependent.

Figure 6

ΔdlaT Mtb (A) or the complemented mutant (ΔdlaT pMV306dlaT) (B) were treated for 4 days with D157070 at pH 5.5 without (black symbols) or with 0.5 mM nitrite (white symbols). Dashed lines denote inocula (6.6 × 107 CFU/ml for ΔdlaT and 5.7 × 107 CFU/ml for ΔdlaT pMV306dlaT). Results in (A) and (B) are mean ± SD of triplicates in single experiments, each of which was repeated at least two times.

(C) Intracellular levels of valine increased in wild-type and ΔdlaT pMV306dlaT Mtb, but not in ΔdlaT, upon treatment with D157070 (10 mM; white bars) as compared to DMSO-treated controls (black bars). Results are mean ± SD from three independent experiments. The following differences in levels of va-line were statistically significant (p < 0.05) by Student's t test: D157070-treated wild-type Mtb versus untreated wild-type; and D157070-treated complemented strain versus untreated complemented strain.