TABLE 2.
Proportion of VAChT+, VGluT2+ and VGAT+ BDA-labeled BF Axonal Varicosities in the LH Region Based on Stereological Estimates1
| VAChT series
|
VGluT2 series
|
VGAT series
|
VTP series
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Cases | BDA +var. | BDA+/VAChT+ var. | % VAChT+ var. | BDA +var. | BDA+/VGluT2+ var. | % VGluT2+ var. | BDA +var. | BDA+/VGAT+ var. | % VGAT +var. | ANOVA F-ratio |
| BDA15-L | 38,080 | 4,640 | 12.2 | - | - | - | - | - | - | |
| BDA15-R | 17,920 | 1,040 | 5.8 | - | - | - | - | - | - | |
| BDA16-L | 18,800 | 2,640 | 14.0 | 27,040 | 5,600 | 20.7 | 23,440 | 11,280 | 48.1 | |
| BDA18-L | 17,840 | 2,000 | 11.2 | 10,800 | 2,080 | 19.3 | 17,680 | 8,240 | 46.6 | |
| BDA18-R | 15,120 | 960 | 6.3 | 11,680 | 2,640 | 22.6 | 17,120 | 8,160 | 47.7 | |
| BDA19-L | - | - | - | 18,824 | 6,136 | 32.6 | 20,176 | 10,504 | 52.1 | |
| BDA19-R | - | - | - | 19,032 | 3,432 | 18.0 | 16,536 | 6,864 | 41.5 | |
| Mean ± SEM | 21,552 ± 4,178 | 2,256 ± 673 | 9.9 ± 1.6 | 17,475 ± 2,946 | 3,978 ± 806 | 22.6 ± 2.6 | 18,990 ± 1,274 | 9,010 ± 816 | 47.2 ± 1.7 | 87.3*** |
1
Data for individual cases (1 or 2 sides from 4 rat brains) is presented together with the mean ± SEM. Estimated numbers of varicosities were obtained by sampling from 3 sections (at levels, A 5800, A 6200 and A 6600), per series per case. The proportions of the three VTP+ varicosities differed significantly (***, P < 0.001 according to one-way ANOVA) across and between VTP+ varicosities (with P < 0.05 according to post-hoc Bonferroni-corrected multiple-comparisons). var., varicosities; VTP, vesicular transporter protein.