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. 2008 Jun 18;3(6):e2471. doi: 10.1371/journal.pone.0002471

Figure 2. sFRP-3 inhibits proliferation induced by EGF.

Figure 2

(A–D) Effect of growth factors on sFRP-3 expressing C3H10T1/2 cells. Growth curves of C3H10T1/2 cells (A) and sFRP-3 expressing C3H10T1/2 (B) cultivated in 2% horse serum (HS)±specific growth factors. Cells grown in 20% FCS or 2% HS alone represented positive and negative control respectively. At the time points indicated, cells were fixed and stained with 0.1% crystal violet solution as described in the Materials and Methods. Dose-dependent effect of EGF on control (C) and sFRP-3 expressing C3H10T1/2 cells (D).