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. 2008 Jun 18;3(6):e2471. doi: 10.1371/journal.pone.0002471

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Scardigli et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–D) sFPR-3 antagonizes the EGF effect rescuing the hair follicle formation in an organ culture of developing mouse skin. Ectoderm explants growth for 48 hours in control medium (A) or in control medium supplied with 7 µg/ml sFRP-3-HA (C), showing normal hair follicles differentiation (black arrows). (B) Ectoderm explants growth in organ culture in medium supplied with 100 ng/ml recombinant EGF for 48 hours, lacking hair follicle differentiation. (D) The co-treatment with EGF and sFPR-3 rescue the hair follicle formation (black arrow).