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. 2008 May 20;9:234. doi: 10.1186/1471-2164-9-234

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Copyright © 2008 Taris et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Outcomes of level of cDNA expression across families for primers (3, 5, 6, 7, 8, 9) technically validated through the analysis of the melting curve of the EST [AJ565694]. From left to right: Plot of PCR cycle number against logarithm PCR product amount; Derivative Melting Curve; Statistical results of level of cDNA expression across families based on a multiple comparison test (Tukey). Family numbers are reported on the horizontal axis, histograms sharing a letter are not significantly different (P < 0.05).