Immunoblot detects β5 integrin expression after
PN7, whereas αv and β3 are expressed at constant adult levels from
birth. In RPE obtained from rats of various ages, β5 integrin was
first detected at PN7 but acquired its adult expression level only at
PN11 (A1, 10 μg protein per lane, A2,
50 μg per lane). β3 and αv integrins were expressed at adult
levels from PN2 (B and C, 50 μg per
lane). RPE-J (J) and ARPE-19 (A19) cell lines displayed similar
integrin expression levels as adult RPE (A2, B, C, 50
μg per lane). Molecular mass standards are indicated in kDa.