Figure 4.

Time course of ROS phagocytosis by RPE-J cells; inhibition by αvβ5 antibody. Internalized fluorescence of samples was measured after trypan blue quenching of extracellular fluorescence. All samples are expressed as percent of total fluorescence after 5 h phagocytosis, which is taken as 100%. Note that practically all fluorescence was extracellular at time points up to 2 h of ROS challenge. In the presence of αvβ5 blocking antibody, total phagocytosis was reduced at all time points (▾), but most intensely at 2 h. Values represent mean ± SD, n = 8.