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β5 integrin (but not β3 integrin) codistributes with internalized ROS. Confocal microscopy sections of RPE-J cells reveal diffuse localization of β5 and β3 integrin when not challenged with ROS (A, β5, and C, β3). After phagocytosis of Texas red-labeled ROS for 5 h, β5 integrin colocalized with phagocytosed ROS, appearing yellow (B). In contrast, in similar samples β3 integrin and ROS derived fluorescence were distinct and the β3 distribution pattern was unaltered (D). Bars = 5 μm.
