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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Am J Physiol Renal Physiol. 2008 Mar 5;294(5):F1157–F1165. doi: 10.1152/ajprenal.00339.2007

Figure 1. Nedd4-2 isoforms differentially regulate ENaC activity in M-1 cells.

Figure 1

Panel A. M-1 cells transduced with adenovirus expressing Nedd4-2, Nedd4-2ΔC2, Nedd4-2ΔWW2,3 or empty virus and Isc measured in Ussing chambers under control conditions and after treatment with 100 nM dexamethasone (dex) for 24 hr. M-1 cells overexpressing all 3 isoforms had significantly lower currents as compared to empty virus (**P < 0.001; Tukey’s pairwise multiple comparison, SigmaStat; n = 15; mean ± SE). Following steroid treatment, currents were significantly different in between each of the Nedd4-2 isoforms (## P < 0.001 compared to Nedd4-2ΔWW2,3; $P < 0.05 compared to Nedd4-2ΔWW2,3; ¶P < 0.05 compared to Nedd4-2; Tukey’s pairwise multiple comparison, SigmaStat; n = 15; mean ± SE). Panel B. Representative western blot of transduced M-1 cells demonstrate relatively equal expression of each of the Nedd4-2 isoforms. Full length Nedd4-2 migrates at ~116 kDa; Nedd4-2ΔC2 (N4-2ΔC2) migrates at ~101 kDa and Nedd4-2ΔWW2,3 (N4-2ΔWW2,3) migrates at ~83 kDa.