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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Am J Physiol Renal Physiol. 2008 Mar 5;294(5):F1157–F1165. doi: 10.1152/ajprenal.00339.2007

Figure 4. Each of the Nedd4-2 isoforms, enhances the ubiquitination of ENaC.

Figure 4

Nedd4-2 isoforms or a control empty vector were transfected with ENaC subunits and HA-tagged ubiquitin (HA-Ub) as indicated into HEK293 cells. ENaC subunits were immunoprecipitated with an anti-FLAG antibody and immunoblotted with anti-HA to detect ubiquitinated ENaC. Total cellular lysates were immunoblotted with anti-FLAG antibody. Ubiquitinated proteins appear as high molecular mass smear. αENaC when expressed alone can be ubiquitinated and ubiquitination of the ENaC complex is increased in the presence of each Nedd4-2 isoform.