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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Am J Physiol Renal Physiol. 2008 Mar 5;294(5):F1157–F1165. doi: 10.1152/ajprenal.00339.2007

Figure 5. The accessory ENaC subunits β and γ are also targets for ubiquitination.

Figure 5

Tagged ENaC subunits were transfected alone or together with or without Nedd4-2 and HA-tagged ubiquitin (HA-Ub) as indicated into HEK293 cells. ENaC subunits were immunoprecipitated with an anti-V5 (β) or anti-myc (γ) antibody and immunoblotted with anti-HA to detect ubiquitinated ENaC subunits. Panel A. ENaC subunit β is ubiquitinated when expressed alone or together and when βENaC is expressed alone this ubiquitination is enhanced by co-expression of Nedd4-2. Panel B. ENaC subunit γ is ubiquitinated when expressed alone or together and as with βENaC this is enhanced by co-expression of Nedd4-2. Similar results were observed from at least three independent experiments for each subunit.