Data are presented as standard histograms for (A) tubulin immunofluorescence and (B) cell body area. (C) Total neurite length profile is presented as a cumulative probability histogram. In all plots, dashed lines represent neurons that were electroporated without plasmid (not transfected, n = 539). Solid lines represent neurons that were electroporated with green fluorescent protein (GFP) cDNA, but were not expressing GFP after 48 h (n = 263). Dotted lines represent GFP-expressing neurons in the same wells (n = 210). Total neurite length was included only for values >10 μm. In the histograms, the y-axis reports percentage of total neurons, normalizing for the different number of cells in the three populations.