Skip to main content
. Author manuscript; available in PMC: 2009 Apr 16.
Published in final edited form as: FEBS Lett. 2008 Mar 25;582(9):1369–1374. doi: 10.1016/j.febslet.2008.03.021

Figure 3.

Figure 3

Reaction profile of diaminopimelate aminotransferase activity in E. coli ATM769 (pDG429) extracts expressing MTH0052 (filled circles) and E. coli ATM769 (pBAD/HisA) vector control extracts (open circles). Cell-free extract (100 μg) was incubated with 4 mM 2-oxoglutarate, 2 mM DL-α,ε-diaminopimelate (racemic), 50 μM pyridoxal 5′-phosphate, 10 mM o-aminobenzaldehyde and 100 mM Tris/HCl (pH 8) in a 300 μl reaction at 37°C. The absorbance of the yellow o-aminobenzaldehyde derivative was measured at 440 nm. Error bars show the standard deviation for three replicates of each sample.