Table 2.

The mRNA levels (mean±SE) of the genes under analysis measured by RT-PCR amplification are reported. Each RT-PCR was repeated at least four times. The semiquantitative analysis of mRNA levels was carried out by the “Gel Doc 2000 UV System” (Bio-Rad, Hercules, CA). The measured mRNA levels were normalised with respect to HPRT (housekeeping gene) and gene expression values were expressed as arbitrary units ± SE.

Gene	Sham	CCI 3 days	CCI/JNJ16259685 3 days	CCI 7 days	CCI/JNJ16259685 7 days
bax/HPRT	0.17±0.03	0.68±0.13*	0.67±0.20*	0.21±0.02	0.18±0.02
bcl-2/HPRT	0.43±0.05	0.36±0.04	1.25±0.18*°	0.71±0.04*	1.34±0.27*°
GFAP/HPRT	5.21±0.31	13.02±0.60*	19.79±0.35°	11.04±1.21*	5.83±0.18°
APAF-1/HPRT	0.63±0.11	1.62±0.32*	0.53±0.02°	0.98±0.13*	0.69±0.10°
caspase-7/HPRT	1.02±0.06	1.64±0.08*	0.84±0.04°	0.83±0.09	1.30±0.06*

^*p<0.05 vs the corresponding sham,

^°p<0.05 vs the CCI JNJ16259685 untreated rats, as analysed by ANOVA, followed by Student-Neuman-Keuls test. JNJ16259685 did not affect behavioural analysis in sham rats. For this reason we did not perform other experiments on JNJ16259685 treatment in sham rats.