Skip to main content
. 2008 Apr 7;111(12):5592–5600. doi: 10.1182/blood-2007-10-113951

Figure 1.

Figure 1

Characterization of MCFD2 missense mutations. (A) MCFD2 missense mutants fail to coimmunoprecipitate with LMAN1. COS1 cells were transfected with myc-tagged WT or mutant MCFD2, metabolically labeled, and immunoprecipitated with the indicated antibodies: myc indicates antimyc; L, anti-LMAN1; and M, anti-MCFD2. (B) MCFD2 missense mutants are mislocalized in cells. HeLa cells were transfected with myc-tagged WT or mutant MCFD2 and stained with rabbit anti-LMAN1, monoclonal antimyc, or monoclonal anti-PDI, and secondary antibodies conjugated with Alexa Fluor 488 or Alexa Fluor 594 (Invitrogen, Carlsbad, CA).