Fig. 5.

Co-localization of Hrs and pTrkB. Immunofluorescence and confocal microscopy of neuronal cultures demonstrated co-localization of Hrs and pTrkB in vesicular structures after 15 min of BDNF treatment (a). This co-localization can be observed in both the cell body (top panels) and in neurite processes (bottom panels). White arrows indicate examples of instances of co-localizations. b, additional immunofluorescence demonstrated co-localization of Hrs, pTrkB, and EEA1 (a marker for early endosomes) after 15 min of BDNF treatment. White arrows in the enlarged image (bottom right panel) indicate locations of triple co-localization.