Table II.
Selected proteins involved in outer segment structure, metabolism, and vesicular trafficking
The number of unique peptides used for the identification is given. The protein to rhodopsin ratio was estimated by APEX analysis. NF, not found in the APEX list of 150 most abundant proteins in the primary data set.
| Protein | Unique peptides | Protein to rhodopsin ratio (APEX) |
|---|---|---|
| Structure | ||
| Peripherin/rds | 14 | 0.141 |
| rom-1 | 11 | 0.248 |
| Prominin-1 | 18 | NF |
| Retinitis pigmentosa 1 protein | 24 | 0.006 |
| Actin 2 | 16 | 0.026 |
| Tubulin, β2 | 20 | 0.128 |
| Metabolism/transport | ||
| Brain creatine kinase | 10 | 0.039 |
| Glucose transporter 1 (GLUT-1) | 7 | 0.038 |
| Glyceraldehyde-3-phosphate dehydrogenase | 25 | 0.109 |
| Hexokinase 1 | 19 | 0.012 |
| Phosphoglycerate kinase 1 | 15 | 0.025 |
| Sodium/potassium-transporting ATPase | 34 | 0.029 |
| Transketolase | 5 | 0.004 |
| Vesicular trafficking | ||
| NSF | 16 | 0.008 |
| Rab 11b | 15 | 0.010 |
| Rab 1b | 10 | 0.030 |
| Rab 18 | 9 | 0.009 |
| Rab 3a | 11 | 0.049 |
| RabGDI (α) | 3 | NF |
| Syntaxin 3 | 8 | 0.013 |
| Syntaxin-binding protein 1 (Munc18-1) | 22 | 0.017 |
| VAMP2/3 | 3 | NF |