Figure 5.

Smad8 rescues the block of Xnot by truncated ALK-2. Albino embryos at the four-cell stage were injected in the marginal zone with in vitro transcribed RNA of ΔALK-2 alone or ΔALK-2 with either Smad2 or Smad8. The embryos were cultured until stage 10.5 and used in whole-mount in situ hybridization with a Xnot probe. The lacZ transcripts was used to indicate the distribution of the injected RNA. Representative embryos are shown (vegetal view with dorsal side up).