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. Author manuscript; available in PMC: 2008 Jun 11.
Published in final edited form as: J Biol Chem. 2007 Sep 26;282(48):35247–35258. doi: 10.1074/jbc.M706782200

Fig. 6.

Fig. 6

m153 forms a homodimer in mammalian cells. A. BiFC analysis. m153 constructs, fused to the N-terminal or C-terminal portions of YFP, were cotransfected in NIH3T3 cells and the live cells were examined by confocal microscopy for fluorescence complementation after 48 hours. As a negative control YFP-fusion constructs of MCMV m144 were cotransfected. Left - YFP channel, Right - overlay of the YFP on the differential interference contrast (DIC) channel. Quantification of the number of YFP positive cells is shown in Figure S2. B. Co-immunoprecipitation of differentially tagged m153. FLAG-m153 (F) or F + HA-m153 (F+HA) constructs were transfected in NIH3T3 cells. 24 hours post transfection post-nuclear cell lysates were immunoprecipitated with anti-FLAG antibodies and detected on a western blot with anti-FLAG (left), anti-m153 rabbit antibodies (middle) or HA-specific antibodies (right).